Nature’s Rite Digestive and Urinary Tonic was tested on Vibrio cholerae, the bacteria that causes cholera, a third-world digestive illness that still ends up being fatal to many people.
EMSL Analytical, Inc.
Microbiology Special Projects Division
8700 Jameel Rd
Suite 190
Houston, TX 77040
(713) 686-3635
Fax (713) 686-3645
Client: Nature’s Rite, LLC
Project: MBC Screening against Vibrio cholerae
EMSL NO: 151401963
Start date: May 1, 2014
Report Date: May 8, 2014
An MBC screening was performed against Vibrio cholerae using the client’s antimicrobial product (Digestive and Urinary Tonic) and test protocol. Testing was performed in triplicate using 5-milliliter (mL) portions of client’s product and 5-mL portions of sterile saline serving as positive controls.
(The method was modified to use sterile saline instead of DIW because the test organism did not survive in the controls using DIW.)
Each portion was inoculated with 100 microliters (uL) of a 1x108 suspension of V. cholerae and incubated for one hour at 35-37 o C. Plate counts were performed after one hour from test and control tubes.
CFU: Colony Forming Units
| Tube | Concentration at one hour (CFU/mL) | Log | Log Reduction | Percent Reduction |
|---|---|---|---|---|
| Test-1 | <10 | <1 | >5.4 | >99.999% |
| Test-2 | <10 | <1 | >5.4 | >99.999% |
| Test-3 | <10 | <1 | >5.4 | >99.999% |
| Control-1 | 2.8 x 106 | 6.4 | ||
| Control-2 | 3.3 x 106 | 6.5 | ||
| Control-3 | 2.1 x 106 | 6.3 | ||
| Control average | 2.7 x 106 | 6.4 |
The test product provided greater than 99.999% reduction of the test bacteria at one hour.
Melanie Rech
Laboratory Director